Recombinant protein purification in Bacteria

37.1 -Production-Par-Bacteries-1-1

Service description

  • Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cells.
  • Proteins can be recombinant, meaning they are generated from an engineered DNA expression vector, which allows for the addition of a tag and higher expression level.
  • In this experiment, the expression is made in a bacterial cell model, which allows for a higher yield of protein purified at a lower cost.

Materials to provide

  • Accession number of protein of interest.
  • Purification conditions if known.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Culture/Transformation.
  • Purification of proteins.
  • Degree of purity.
  • Frozen protein in aliquots.
  • Gel picture after purification.
  • Quantification.
  • Full disclosure of the purification process.
  • Study following the specifications validated with the Expert.

Get started with your recombinant protein purification project

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