Recombinant protein purification in Bacteria

37.1 -Production-Par-Bacteries

Service description

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cells. Proteins can be recombinant, meaning they are generated from an engineered DNA expression vector, which allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a bacterial cell model, which allows for a higher yield of protein purified at a lower cost.

Materials to provide

  • Accession number of protein of interest
  • Purification conditions if known

    Further details on the project may be requested.

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Typical deliverables

  • Culture/Transformation
  • Purification of proteins
  • Degree of purity
  • Frozen protein in aliquots
  • Gel picture after purification
  • Quantification
  • Full disclosure of the purification process
  • Study following the specifications validated with the Expert

Get started with your recombinant protein purification project

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