Recombinant proteins production in insect cell model

36.1 - Production-CellulesInsectes

Service description

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cell lysates. Proteins can be recombinant, meaning they are generated from engineered DNA expression vector, that allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a insect cell model, which typically allows for post-translational modifications and in some cases a better folding of the protein.

Materials to provide

  • Sequence of the protein to express
  • Bacmid or low-titer viral stock
  • Expression conditions

    Further details on the project may be requested.

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Typical deliverables

  • Culture/Infection/Transfection
  • Purification of proteins
  • Degree of purity
  • Frozen protein in aliquots
  • Gel picture
  • Quantification
  • Full disclosure of the purification process
  • Study following the specifications validated with the Expert

Get started with your recombinant protein purification project

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