Recombinant proteins production in insect-cell model
Service description
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cell lysates.
Proteins can be recombinant, meaning they are generated from an engineered DNA expression vector, that allows for the addition of a tag and higher expression level.
In this experiment, the expression is made in an insect cell model, which typically allows for post-translational modifications and in some cases a better folding of the protein.
Materials to provide
Sequence of the protein to express.
Bacmid or low-titer viral stock.
Expression conditions.
Further details on the project may be requested.
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Typical deliverables
Culture/Infection/Transfection.
Purification of proteins.
Degree of purity.
Frozen protein in aliquots.
Gel picture.
Quantification.
Full disclosure of the purification process.
Study following the specifications validated with the Expert.
Get started with your recombinant protein purification project
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