Recombinant proteins production in insect cell model
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cell lysates. Proteins can be recombinant, meaning they are generated from engineered DNA expression vector, that allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a insect cell model, which typically allows for post-translational modifications and in some cases a better folding of the protein.
Materials to provide
- Sequence of the protein to express
- Bacmid or low-titer viral stock
- Expression conditions
Further details on the project may be requested.
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- Purification of proteins
- Degree of purity
- Frozen protein in aliquots
- Gel picture
- Full disclosure of the purification process
- Study following the specifications validated with the Expert
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