In vitro imaging: Microscopy


Service description

  • Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cells.
  • Proteins can be recombinant, meaning they are generated from an engineered DNA expression vector, that allows for the addition of a tag and higher expression level.
  • In this experiment, the expression is made in a mammalian cell model, which allows for extensive post-translational modifications and appropriate folding of the expressed protein.

Materials to provide

  • Sequence of the protein to express.
  • Expression vector and its map.
  • Expression conditions.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Culture/Infection/Transfection.
  • Expression and purification of proteins.
  • Degree of purity as requested.
  • Frozen protein in aliquots.
  • Gel picture after purification.
  • Quantification.
  • Full disclosure of the purification process.
  • Study following the specifications validated with the Expert.

Get started with your recombinant protein purification project

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