Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cells.
Proteins can be recombinant, meaning they are generated from an engineered DNA expression vector, that allows for the addition of a tag and higher expression level.
In this experiment, the expression is made in a mammalian cell model, which allows for extensive post-translational modifications and appropriate folding of the expressed protein.
Materials to provide
Sequence of the protein to express.
Expression vector and its map.
Expression conditions.
Further details on the project may be requested.
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Typical deliverables
Culture/Infection/Transfection.
Expression and purification of proteins.
Degree of purity as requested.
Frozen protein in aliquots.
Gel picture after purification.
Quantification.
Full disclosure of the purification process.
Study following the specifications validated with the Expert.
Get started with your recombinant protein purification project
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