38.1 - Production-CellulesMammiferes

Service description

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cells. Proteins can be recombinant, meaning they are generated from engineered DNA expression vector, that allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a mammalian cell model, which allows for extensive post-translational modifications and appropriate folding of the expressed protein.

Materials to provide

  • Sequence of the protein to express
  • Expression vector and its map
  • Expression conditions

    Further details on the project may be requested.

You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Culture/Infection/Transfection
  • Expression and purification of proteins
  • Degree of purity as requested
  • Frozen protein in aliquots
  • Gel picture after purification
  • Quantification
  • Full disclosure of the purification process
  • Study following the specifications validated with the Expert

Get started with your recombinant protein purification project

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