Heterogeneity of transcriptome can be quite high in a cell population, thus Single-cell RNA sequencing (scRNA-Seq) combines the isolation of a single cell with powerful Next-generation sequencing techniques, making the identification of rare cell types within a population possible.
scRNA-Seq requires a reverse transcription of RNA after cell isolation, followed by amplification before sequencing.
Materials to provide
Samples.
Proof of sample quality or integrity (Bioanalyzer traces or eq.).
Further details on the project may be requested.
You will be able to exchange directly with the experts after filling in the form below.
Typical deliverables
Extraction.
Library preparation.
Sequencing of the samples with the requested number of reads.
Paired-end reads.
Quantification analysis.
Download the link or a hard drive with raw data and the post-experiment analysis.
Study following the specifications validated with the Expert.
Get started with your single-cell RNA sequencing project
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