Stable cell line generation is a set of techniques that allows for the long-term expression or inhibition of a gene of interest in a eukaryotic cell.
Culture cells are transfected by linear or circular DNA and plated. Cells are then isolated into separated wells, selected for the presence of the transfected DNA (typically using antibiotics), and analyzed for the expression of the gene of interest.
Materials to provide
Primary cells / Cell lines.
Culture conditions.
Further details on the project may be requested.
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Typical deliverables
Stable clones generation.
Multiple clones included per sample.
Verification by qPCR and/or Western blot.
Frozen vials of stable cells.
Culture conditions.
Study following the specifications validated with the Expert.
Get started with your stable cell line development project
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