Stable cell lines development

17.1 - LigneeStable

Service description

Stable cell line generation is a set of technique that allows for the long-term expression or inhibition of a gene of interest in an eukaryotic cell. Culture cells are transfected by linear or circular DNA and plated. Cells are then isolated into separated wells, selected for the presence of the transfected DNA (typically using antibiotics) and analyzed for the expression of the gene of interest.

Materials to provide

  • Primary cells / Cell lines
  • Culture conditions

    Further details on the project may be requested.

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Typical deliverables

  • Stable clones generation
  • Multiple clones included per sample
  • Verification by qPCR and/or Western blot
  • Frozen vials of stable cells
  • Culture conditions
  • Study following the specifications validated with the Expert

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