Whole Transcriptome Shotgun Sequencing (WTSS) allows for the detection, sequence, and quantification of all RNA in a given biological sample.
Typical applications of RNA sequencing include analysis of changes in the transcriptome (i.e. differences in gene expression), post-transcriptional modifications, and detection of mutations.
In addition to mRNA, non-coding RNA and short RNA are also sequenced with WTSS.
Materials to provide
Samples.
Proof of sample quality or integrity (Bioanalyzer traces or eq.).
Further details on the project may be requested.
You will be able to exchange directly with the experts after filling in the form below.
Typical deliverables
Extraction.
Library preparation.
Depletion.
Sequencing of the samples with the number of reads.
Paired-end reads.
Download the link or a hard drive with raw data and the post-experiment analysis.
Study following the specifications validated with the Expert.
Get started with your whole transcriptome next-generation sequencing project
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