Whole transcriptome shotgun sequencing
Whole Transcriptome Shotgun Sequencing (WTSS) allows for the detection, the sequence and the quantification of all RNA in a given biological sample. Typical applications of RNA sequencing include analysis of changes in transcriptome (i.e. differences in gene expression), post-transcriptional modifications and detection of mutations. In addition to mRNA, non-coding RNA and short RNA are also sequenced with WTSS.
Materials to provide
- Proof of samples quality or integrity (Bioanalyzer traces or eq.)
Further details on the project may be requested.
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- Library preparation
- Sequencing of the samples with number of reads
- Paired-end reads
- Download link or a harddrive with raw data and the post-experiment analysis
- Study following the specifications validated with the Expert
Get started with your whole transcriptome next-generation sequencing project
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