Copy number variation analysis (CNV)

CRISP-R 300x225

Service description

  • CRISPR/Cas9 is a powerful tool for genome editing. It requires the construction of a DNA vector that codes both for Cas9 protein and a target-specific RNA called crRNA (also called single-guide RNA when combined with another sequence, tracrRNA) that must bind only where editing is desirable.
  • In this experiment, the Expert will design and construct the DNA vector that should allow for the edition of the sequence in the target cell's DNA.

Materials to provide

  • Accession number of genes of interest.
  • Cell lines to transfect.
  • Antibodies and conditions.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Bacterial clones and preparation of the guide vector with insert.
  • Map of the vector.
  • Donor vector.
  • Cell line edition and shipping cell lines in frozen vials (3 x 1ml).
  • Data for sorting enrichment.
  • Gene knock-out PCR verification if cell selection is performed.
  • Monoallelic verification.
  • Western blot data.
  • Removal of the selection marker.
  • Study following the specifications validated with the Expert.
Capture d’écran 2020-10-16 à 10.57.43

Get started with your CRISPR/Cas9 cell line edition

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