Service description
2D electrophoresis allows the separation and quantification of whole proteins, in contrast to bottom-up LC-MS/MS approaches in which proteins are digested into peptides. This separative method can be used to visualize the profile of a moderately complex protein extract or to detect different isoforms of the same protein (post-translational modifications). Qualitative or quantitative analysis can be performed with the analysis of gel images using dedicated software.
Materials to provide
Protein extracts in a buffer adapted to the 2D-DIGE approachFurther details on the project may be requested.
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Typical deliverables
- List of significant variant spots (Excel format)
- All images and quantification data can be transmitted on request.
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