Sub-cloning corresponds to a molecular cloning in which the gene of interest is integral to a fragment that can be simply cut and paste into a new plasmid vector. This process is an alternative to a DNA amplification of the gene prior to its insertion in a vector DNA.
Materials to provide
Sequence inserted in the origin plasmid
Map of the plasmids
Target plasmid
Further details on the project may be requested.
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Typical deliverables
Bacterial clone on paper, glycerol or agar
Prep of purified DNA
Sequencing of the insert
Map of the plasmid with cloning strategy
Study following the specifications validated with the Expert
Get started with your plasmid subcloning
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