NGS Ribosomal profiling


Service description

  • Ribosome profiling or Ribo-seq is a technique that allows for the determination of actively translated mRNA in a sample.
  • Using an initial treatment of ribonuclease followed by a protease treatment and sequencing, Ribo-seq provides the exact location of ribosomes on mRNA, thus enabling the identification of translation start sites, the complement of translated ORFs in a cell or tissue, the distribution of ribosomes on an mRNA, and the speed of translation.

Materials to provide

  • Samples.
  • RNase treatment information.
  • Proof of sample quality or integrity (Bioanalyzer traces or eq.).

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Extraction.
  • Library preparation.
  • Depletion.
  • RNase treatment if the library is not ready.
  • Sequencing of the samples with a number of reads.
  • Paired-end reads.
  • Download the link or a hard drive with raw data, activity data analysis, and advanced analytics.
  • Study following the specifications validated with the Expert.

Get started with your ribosomal profiling project

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