Ribosome profiling or Ribo-seq is a technique that allows for the determination of actively translated mRNA in a sample.
Using an initial treatment of ribonuclease followed by a protease treatment and sequencing, Ribo-seq provides the exact location of ribosomes on mRNA, thus enabling the identification of translation start sites, the complement of translated ORFs in a cell or tissue, the distribution of ribosomes on an mRNA, and the speed of translation.
Materials to provide
Samples.
RNase treatment information.
Proof of sample quality or integrity (Bioanalyzer traces or eq.).
Further details on the project may be requested.
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Typical deliverables
Extraction.
Library preparation.
Depletion.
RNase treatment if the library is not ready.
Sequencing of the samples with a number of reads.
Paired-end reads.
Download the link or a hard drive with raw data, activity data analysis, and advanced analytics.
Study following the specifications validated with the Expert.
Get started with your ribosomal profiling project
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