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2D-electrophoresis for quantification

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Service description

  • Separation and quantification of whole proteins, in contrast to bottom-up LC-MS/MS approaches in which proteins are digested into peptides.
  • Visualize the profile of a moderately complex protein extract.
  • Detect different isoforms of the same protein (post-translational modifications).
  • Qualitative or quantitative analysis can be performed with the analysis of gel images using dedicated software.

Materials to provide

  • Protein extracts in a buffer adapted to the 2D-DIGE approach.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • List of significant variant spots (Excel format)
  • All images and quantification data can be transmitted on request.
Capture d’écran 2020-10-16 à 10.57.43

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