2D electrophoresis allows the separation and quantification of whole proteins, in contrast to bottom-up LC-MS/MS approaches in which proteins are digested into peptides. This separative method can be used to visualize the profile of a moderately complex protein extract or to detect different isoforms of the same protein (post-translational modifications). Qualitative or quantitative analysis can be performed with the analysis of gel images using dedicated software.
Materials to provideProtein extracts in a buffer adapted to the 2D-DIGE approach
Further details on the project may be requested.
You will be able to exchange directly with the experts after filling in the form below.
- List of significant variant spots (Excel format)
- All images and quantification data can be transmitted on request.
Get started with your 2D-electrophoresis project
Please answer the fields below to be contacted by our team of scientists