Site-directed mutagenesis is a set of techniques that allows the specific swapping, the deletion, or the addition of one or a few nucleotides in a double-stranded DNA sequence.
It requires the usage of PCR amplification of the sequence using primers containing the alteration of the sequence.
The amplified fragment can be reintegrated into a new vector, or the entire vector can be sequenced for verification of integrity.
Materials to provide
Original sequence to mutate in the expression vector.
Map of the origin plasmid.
Sequence/accession number of the gene to mutate.
Further details on the project may be requested.
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Typical deliverables
Bacterial clone on paper, glycerol or agar.
Purified DNA.
Sequencing of the sequence of interest.
Report.
Study following the specifications validated with the Expert.
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