Plasmid site-directed mutagenesis

45.1o - VecteurMutagenese 300X169

Service description

  • Site-directed mutagenesis is a set of techniques that allows the specific swapping, the deletion, or the addition of one or a few nucleotides in a double-stranded DNA sequence.
  • It requires the usage of PCR amplification of the sequence using primers containing the alteration of the sequence.
  • The amplified fragment can be reintegrated into a new vector, or the entire vector can be sequenced for verification of integrity.

Materials to provide

  • Original sequence to mutate in the expression vector.
  • Map of the origin plasmid.
  • Sequence/accession number of the gene to mutate.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Bacterial clone on paper, glycerol or agar.
  • Purified DNA.
  • Sequencing of the sequence of interest.
  • Report.
  • Study following the specifications validated with the Expert.
Capture d’écran 2020-10-16 à 10.57.43

Get started with your site-directed mutagenesis request

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