Plasmid site-directed mutagenesis

45.1 - VecteurMutagenese

Service description

Site-directed mutagenesis is a set of techniques that allows the specific swapping, the deletion or the addition of one or a few nucleotides in a double stranded DNA sequence. It requires the usage of a PCR amplification of the sequence using primers containing the alteration of the sequence. Amplified fragment can be reintegrated into a new vector, or the entire vector can be sequenced for verification of integrity.

Materials to provide

  • Original sequence to mutate in expression vector
  • Map of the origin plasmid
  • Sequence/accession number of the gene to mutate

    Further details on the project may be requested.

You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Bacterial clone on paper, glycerol or agar
  • Purified DNA
  • Sequencing of the sequence of interest
  • Report
  • Study following the specifications validated with the Expert

Get started with your site-directed mutagenesis request

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