Plasmid site-directed mutagenesis

45.1 - VecteurMutagenese

Service description

Site-directed mutagenesis is a set of techniques that allows the specific swapping, the deletion or the addition of one or a few nucleotides in a double stranded DNA sequence. It requires the usage of a PCR amplification of the sequence using primers containing the alteration of the sequence. Amplified fragment can be reintegrated into a new vector, or the entire vector can be sequenced for verification of integrity.

Materials to provide

  • Original sequence to mutate in expression vector
  • Map of the origin plasmid
  • Sequence/accession number of the gene to mutate

    Further details on the project may be requested.

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Typical deliverables

  • Bacterial clone on paper, glycerol or agar
  • Purified DNA
  • Sequencing of the sequence of interest
  • Report
  • Study following the specifications validated with the Expert

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