Plasmid site-directed mutagenesis
Site-directed mutagenesis is a set of techniques that allows the specific swapping, the deletion or the addition of one or a few nucleotides in a double stranded DNA sequence. It requires the usage of a PCR amplification of the sequence using primers containing the alteration of the sequence. Amplified fragment can be reintegrated into a new vector, or the entire vector can be sequenced for verification of integrity.
Materials to provide
- Original sequence to mutate in expression vector
- Map of the origin plasmid
- Sequence/accession number of the gene to mutate
Further details on the project may be requested.
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- Bacterial clone on paper, glycerol or agar
- Purified DNA
- Sequencing of the sequence of interest
- Study following the specifications validated with the Expert
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