Gene expression analysis by qPCR using dyes


Service description

  • Quantitative PCR (qPCR) with probes is a powerful technique that combines the amplification of a specific DNA fragment using PCR with the highly-sensitive detection of fluorescence in the sample using specific probes.
  • The more amplification of DNA in the tube, the more probes will be integrated to the amplified DNA, releasing a fluorophore from quenching in the process and thus allowing for a quantitative determination of initial specific DNA in the sample.

Materials to provide

  • Samples in sufficient amounts.
  • Primers and probes, or accession numbers of the gene of interest.
  • Description of the samples and QC data.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Extracts.
  • RT-PCR.
  • QC of extracts.
  • NTC or NAC controls.
  • qPCR experiment in replicates.
  • Controls.
  • Relative quantification.
  • Data analysis.
  • Study following the specifications validated with the Expert.

Get started with your qPCR gene expression analysis project

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