Quantitative PCR (qPCR) with probes is a powerful technique that combines the amplification of a specific DNA fragment using PCR with the highly-sensitive detection of fluorescence in the sample using specific probes.
The more amplification of DNA in the tube, the more probes will be integrated to the amplified DNA, releasing a fluorophore from quenching in the process and thus allowing for a quantitative determination of initial specific DNA in the sample.
Materials to provide
Samples in sufficient amounts.
Primers and probes, or accession numbers of the gene of interest.
Description of the samples and QC data.
Further details on the project may be requested.
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Typical deliverables
Extracts.
RT-PCR.
QC of extracts.
NTC or NAC controls.
qPCR experiment in replicates.
Controls.
Relative quantification.
Data analysis.
Study following the specifications validated with the Expert.
Get started with your qPCR gene expression analysis project
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