Gene expression analysis by qPCR using dyes

5 - Gene Expression

Service description

Quantitative PCR (qPCR) with probes is a powerful technique that combines the amplification of a specific DNA fragment using PCR with the highly-sensitive detection of fluorescence in the sample using specific probes. The more amplification of DNA in the tube, the more probes will be integrated to the amplified DNA, releasing a fluorophore from quenching in the process and thus allowing for a quantitative determination of initial specific DNA in the sample.

Materials to provide

  • Samples in sufficient amount
  • Primers and probes, or accession number of the gene of interest
  • Description of the samples and QC data

    Further details on the project may be requested.

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Typical deliverables

  • Extracts
  • RT-PCR
  • QC of extracts
  • NTC or NAC controls
  • qPCR experiment in replicates
  • Controls
  • Relative quantification
  • Data analysis
  • Study following the specifications validated with the Expert

Get started with your qPCR gene expression analysis project

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